Standardized Brain Block (SBB) and Standardized Cortex/White matter Vial (SCxV/SWmV) ( Vonsattel et al. 2007).Ī central aspect of this endeavor at New York Brain Bank (NYBB) has been changing the paradigm from freezing the donated brain en bloc pending future dissection to processing of the brain immediately at fresh state and storing frozen samples of standardized dimensions and contents, i.e. 1992 Alafuzoff and Winblad 1993 Grinberg et al. The infrastructure and logistics of providing postmortem human central nervous system tissue is a rapidly evolving field that increasingly receives attention and funding ( Ravid et al. 1995), to unravel the mechanisms underlying dementia, movement disorders or psychiatric diseases ( Grinberg et al.
Insight into the pathogenesis of neurodegenerative disorders exclusively occurring in humans requires accurately categorized postmortem human brains, including brains of healthy control subjects ( Stopa and Bird 1989 Ravid et al. The NYBB system of brain banking has the following key advantages: (1) The dissection of the brain and the harvest of samples at the fresh state improve their anatomic specificity and quality (2) samples are ready for immediate disbursement once categorized diagnostically, reducing the time between the receipt of request and disbursement of samples (3) the methods prevent thaw-refreeze cycles and carving out of regions of interest from frozen tissue, which is cumbersome and deleterious to the both samples and source brains (4) accurate quantitative data on stored samples according to anatomical regions and distributive diagnosis guides future sample collection and fosters effective use of limited resources. Within the same time period, 1,094 requests were processed and 9,096 samples were disbursed with an average turnaround time of five working days. In the 5 years since the inception of NYBB (2002-2007) 560 brains (63,252 fresh frozen samples) were processed and as of 11/2007, 54,242 samples are stored seven freezers occupying 81% of maximum capacity of NYBB. NYBB tissue tracking uses a relational database to co-register a bar coded, unique sample identifier to unique coordinates in the three-dimensional freezer space, allowing immediate retrieval of stored samples without further dissection. This article introduces electronic tissue tracking and management as implemented at New York Brain Bank (NYBB) through processing of the brain at fresh state and storing standardized frozen samples. Insight into the pathogenesis of neurodegenerative disorders requires accurately categorized postmortem human brain tissue.
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